MersV1, Main, Exploration, bibRecord, 004362

BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax

Identifieur interne : 004362 ( Main/Exploration ); précédent : 004361; suivant : 004363

BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax

Auteurs : Xiao-Ming Yin [États-Unis] ; Zoltn N. Oltvai [États-Unis] ; Stanley J. Korsmeyer [États-Unis]

Source :

Nature [ 0028-0836 ] ; 1994-05-26.

RBID : ISTEX:A1727DCD54080C32F4439657AA8D5F4F6E23D7D5

Abstract

BCL-2 was isolated from the t(14;18) chromosomal breakpoint in follicular B-cell lymphoma13. Bcl-2 has the unique oncogenic role of extending cell survival by inhibiting a variety of apoptotic deaths413. An emerging family of Bcl-2 -related proteins share two highly conserved regions1420 referred to here as Bcl-2 homology 1 and 2 (BH1 and BH2) domains (Fig. 1). This includes Bax which heterodimerizes with Bcl-2 and when overexpressed counteracts Bcl-214. We report here that site-specific mutagenesis of Bcl-2 establishes the two domains as novel dimerization motifs. Substitu-tion of Gly 145 in BHl domain or Trp 188 in BH2 domain completely abrogated Bcl-2's death-repressor activity in inter-leukin-3 deprivation, -irradiation and glucocorticoid-induced apoptosis. Mutations that affected Bcl-2's function also disrupted its heterodimerization with Bax, yet still permitted Bcl-2 homo-dimerization. These results establish a functional role for the BH1 and BH2 domains and suggest Bcl-2 exerts its action through heterodimerization with Bax.

Url:

https://api.istex.fr/ark:/67375/GT4-DRFVGZZW-C/fulltext.pdf

DOI: 10.1038/369321a0

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 002144
to stream Istex, to step Curation: 002144
to stream Istex, to step Checkpoint: 001999
to stream Main, to step Merge: 004425
to stream Main, to step Curation: 004362

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax</title>
<author><name sortKey="Yin, Xiao Ming" sort="Yin, Xiao Ming" uniqKey="Yin X" first="Xiao-Ming" last="Yin">Xiao-Ming Yin</name>
</author>
<author><name sortKey="Oltvai, Zoltn N" sort="Oltvai, Zoltn N" uniqKey="Oltvai Z" first="Zoltn N." last="Oltvai">Zoltn N. Oltvai</name>
</author>
<author><name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A1727DCD54080C32F4439657AA8D5F4F6E23D7D5</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1038/369321a0</idno>
<idno type="url">https://api.istex.fr/ark:/67375/GT4-DRFVGZZW-C/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002144</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002144</idno>
<idno type="wicri:Area/Istex/Curation">002144</idno>
<idno type="wicri:Area/Istex/Checkpoint">001999</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001999</idno>
<idno type="wicri:doubleKey">0028-0836:1994:Yin X:bh:and:bh</idno>
<idno type="wicri:Area/Main/Merge">004425</idno>
<idno type="wicri:Area/Main/Curation">004362</idno>
<idno type="wicri:Area/Main/Exploration">004362</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax</title>
<author><name sortKey="Yin, Xiao Ming" sort="Yin, Xiao Ming" uniqKey="Yin X" first="Xiao-Ming" last="Yin">Xiao-Ming Yin</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Molecular Oncology, Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, Missouri 63110</wicri:regionArea>
<orgName type="university">École de médecine (Université Washington de Saint-Louis)</orgName>
<placeName><settlement type="city">Saint-Louis (Missouri)</settlement>
<region type="state">Missouri (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Oltvai, Zoltn N" sort="Oltvai, Zoltn N" uniqKey="Oltvai Z" first="Zoltn N." last="Oltvai">Zoltn N. Oltvai</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Molecular Oncology, Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, Missouri 63110</wicri:regionArea>
<orgName type="university">École de médecine (Université Washington de Saint-Louis)</orgName>
<placeName><settlement type="city">Saint-Louis (Missouri)</settlement>
<region type="state">Missouri (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
<affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Division of Molecular Oncology, Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, Missouri 63110</wicri:regionArea>
<orgName type="university">École de médecine (Université Washington de Saint-Louis)</orgName>
<placeName><settlement type="city">Saint-Louis (Missouri)</settlement>
<region type="state">Missouri (État)</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Nature</title>
<imprint><publisher>Nature Publishing Group</publisher>
<date when="1994-05-26">1994-05-26</date>
<biblScope unit="vol">369</biblScope>
<biblScope unit="issue">6478</biblScope>
<biblScope unit="page" from="321">321</biblScope>
<biblScope unit="page" to="323">323</biblScope>
<date type="Copyright" when="1994">1994</date>
</imprint>
<idno type="ISSN">0028-0836</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0028-0836</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">BCL-2 was isolated from the t(14;18) chromosomal breakpoint in follicular B-cell lymphoma13. Bcl-2 has the unique oncogenic role of extending cell survival by inhibiting a variety of apoptotic deaths413. An emerging family of Bcl-2 -related proteins share two highly conserved regions1420 referred to here as Bcl-2 homology 1 and 2 (BH1 and BH2) domains (Fig. 1). This includes Bax which heterodimerizes with Bcl-2 and when overexpressed counteracts Bcl-214. We report here that site-specific mutagenesis of Bcl-2 establishes the two domains as novel dimerization motifs. Substitu-tion of Gly 145 in BHl domain or Trp 188 in BH2 domain completely abrogated Bcl-2's death-repressor activity in inter-leukin-3 deprivation, -irradiation and glucocorticoid-induced apoptosis. Mutations that affected Bcl-2's function also disrupted its heterodimerization with Bax, yet still permitted Bcl-2 homo-dimerization. These results establish a functional role for the BH1 and BH2 domains and suggest Bcl-2 exerts its action through heterodimerization with Bax.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Missouri (État)</li>
</region>
<settlement><li>Saint-Louis (Missouri)</li>
</settlement>
<orgName><li>École de médecine (Université Washington de Saint-Louis)</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Yin, Xiao Ming" sort="Yin, Xiao Ming" uniqKey="Yin X" first="Xiao-Ming" last="Yin">Xiao-Ming Yin</name>
</region>
<name sortKey="Korsmeyer, Stanley J" sort="Korsmeyer, Stanley J" uniqKey="Korsmeyer S" first="Stanley J." last="Korsmeyer">Stanley J. Korsmeyer</name>
<name sortKey="Oltvai, Zoltn N" sort="Oltvai, Zoltn N" uniqKey="Oltvai Z" first="Zoltn N." last="Oltvai">Zoltn N. Oltvai</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004362 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004362 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:A1727DCD54080C32F4439657AA8D5F4F6E23D7D5
   |texte=   BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax

BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri